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cellbrite steady 488  (Biotium)


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    Structured Review

    Biotium cellbrite steady 488
    Cellbrite Steady 488, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellbrite+steady+488/CellBrite+Steady+405+Membrane+Staining+Kit/pm41045193-342-62-65
    Average 94 stars, based on 232 article reviews
    cellbrite steady 488 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Peptide Amphiphiles Hitchhike on Endogenous Biomolecules for Enhanced Cancer Imaging and Therapy.
    Article Snippet: Materials: Peptides were commercially obtained from Genscript with purity >95%. .. The following reagents were used: ICG maleimide (Iris Biotech, RL-2820), ICG (Fisher Scientific, I0535100MG), Cy5 maleimide (BroadPharm, BP-22552), maleimide (Sigma-Aldrich, 129 585), vcMMAE (MedChem Express, HY-15575), MMAE (MedChem Express, HY15162), HDL from human plasma (Sigma-Aldrich, SAE0054), LDL from human plasma (Sigma-Aldrich, SAE0053), VLDL from human plasma (Millipore Sigma, 437 647), human serum albumin (Fisher Scientific, OSRHSA10), pooled human plasma (Innovative Research, IPLAWBK2E50ML), CellBrite Steady 488 (Biotium, 30106-T), FITC-dextran 70 (Santa Cruz Biotechnology, sc-263323), brefeldin A (Alfa Aesar, J62340), bromsulphalein (MedChem Express, HY-D0217), chlorpromazine hydrochloride (Sigma-Aldrich, C8138), cis-ACBD (Tochris, 0271), filipin complex (Sigma-Aldrich, F9765), heparin sodium salt (Sigma-Aldrich, H3149-10KU), methyl-β-cyclodextrin (Sigma-Aldrich, C4555-5G), wortmannin (Alfa Aesar, J63983), bovine serum albumin (VWR, 0332), 4′,6- diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, D9542), Hoechst stain (Invitrogen, H3570), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) cell proliferation assay (Abcam, ab197010). .. Gadodiamide (GdDTPA-BMA, Omniscan, GE Healthcare Inc. Marlborough, MA) MRI contrast was purchased through the OHSU in-patient pharmacy.

    Staining:

    Article Title: Peptide Amphiphiles Hitchhike on Endogenous Biomolecules for Enhanced Cancer Imaging and Therapy.
    Article Snippet: Materials: Peptides were commercially obtained from Genscript with purity >95%. .. The following reagents were used: ICG maleimide (Iris Biotech, RL-2820), ICG (Fisher Scientific, I0535100MG), Cy5 maleimide (BroadPharm, BP-22552), maleimide (Sigma-Aldrich, 129 585), vcMMAE (MedChem Express, HY-15575), MMAE (MedChem Express, HY15162), HDL from human plasma (Sigma-Aldrich, SAE0054), LDL from human plasma (Sigma-Aldrich, SAE0053), VLDL from human plasma (Millipore Sigma, 437 647), human serum albumin (Fisher Scientific, OSRHSA10), pooled human plasma (Innovative Research, IPLAWBK2E50ML), CellBrite Steady 488 (Biotium, 30106-T), FITC-dextran 70 (Santa Cruz Biotechnology, sc-263323), brefeldin A (Alfa Aesar, J62340), bromsulphalein (MedChem Express, HY-D0217), chlorpromazine hydrochloride (Sigma-Aldrich, C8138), cis-ACBD (Tochris, 0271), filipin complex (Sigma-Aldrich, F9765), heparin sodium salt (Sigma-Aldrich, H3149-10KU), methyl-β-cyclodextrin (Sigma-Aldrich, C4555-5G), wortmannin (Alfa Aesar, J63983), bovine serum albumin (VWR, 0332), 4′,6- diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, D9542), Hoechst stain (Invitrogen, H3570), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) cell proliferation assay (Abcam, ab197010). .. Gadodiamide (GdDTPA-BMA, Omniscan, GE Healthcare Inc. Marlborough, MA) MRI contrast was purchased through the OHSU in-patient pharmacy.

    Proliferation Assay:

    Article Title: Peptide Amphiphiles Hitchhike on Endogenous Biomolecules for Enhanced Cancer Imaging and Therapy.
    Article Snippet: Materials: Peptides were commercially obtained from Genscript with purity >95%. .. The following reagents were used: ICG maleimide (Iris Biotech, RL-2820), ICG (Fisher Scientific, I0535100MG), Cy5 maleimide (BroadPharm, BP-22552), maleimide (Sigma-Aldrich, 129 585), vcMMAE (MedChem Express, HY-15575), MMAE (MedChem Express, HY15162), HDL from human plasma (Sigma-Aldrich, SAE0054), LDL from human plasma (Sigma-Aldrich, SAE0053), VLDL from human plasma (Millipore Sigma, 437 647), human serum albumin (Fisher Scientific, OSRHSA10), pooled human plasma (Innovative Research, IPLAWBK2E50ML), CellBrite Steady 488 (Biotium, 30106-T), FITC-dextran 70 (Santa Cruz Biotechnology, sc-263323), brefeldin A (Alfa Aesar, J62340), bromsulphalein (MedChem Express, HY-D0217), chlorpromazine hydrochloride (Sigma-Aldrich, C8138), cis-ACBD (Tochris, 0271), filipin complex (Sigma-Aldrich, F9765), heparin sodium salt (Sigma-Aldrich, H3149-10KU), methyl-β-cyclodextrin (Sigma-Aldrich, C4555-5G), wortmannin (Alfa Aesar, J63983), bovine serum albumin (VWR, 0332), 4′,6- diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, D9542), Hoechst stain (Invitrogen, H3570), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) cell proliferation assay (Abcam, ab197010). .. Gadodiamide (GdDTPA-BMA, Omniscan, GE Healthcare Inc. Marlborough, MA) MRI contrast was purchased through the OHSU in-patient pharmacy.

    Isolation:

    Article Title: Host extracellular vesicles confer cytosolic access to systemic LPS licensing non-canonical inflammasome sensing and pyroptosis.
    Article Snippet: Intracellular surveillance for systemic microbial components during homeostasis and infections governs host physiology and immunity.. However, a long-standing question is how circulating microbial ligands become accessible to intracellular receptors.. Here we show a role for host-derived extracellular vesicles (EVs) in this process; human and murine plasma-derived and cell culture-derived EVs have an intrinsic capacity to bind bacterial lipopolysaccharide (LPS).



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    cellbrite steady 488  (Biotium)
    94
    Biotium cellbrite steady 488
    Cellbrite Steady 488, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellbrite+steady+488/CellBrite+Steady+405+Membrane+Staining+Kit/pm41045193-342-62-65
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    cellbrite steady 488 - by Bioz Stars, 2026-10
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    cellbrite steady 488 membrane staining kit  (Biotium)
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    Biotium cellbrite steady 488 membrane staining kit
    EGFR/c-Met bsApt stains the cell surface and is internalized into H1975 cells Confocal microscopy images of H1975 cells stained upon incubation with 150 nM Atto647N-labeled aptamer samples: C36 (non-targeting control RNA aptamer), MinE07 (anti-EGFR aptamer), CLN3 (anti-c-Met aptamer), or EGFR/c-Met bsApt (bsApt). Cells were also stained with CellBrite <t>Steady</t> <t>488</t> (cell membrane stain) and Hoechst 33342 (nuclear marker stain). Representative images from one of n = 2 independent experiments are shown: Atto647N-aptamers (red, excitation 647 nm/emission 657–692 nm), cell membrane (green, excitation 488 nm/emission 521–551 nm), nuclei (blue, excitation 405 nm/emission 436–473 nm). Scale bars, 20 μm. Qualitatively, MinE07 was internalized after 45-min incubation at 37°C and found predominantly in the cytosol, as it did not show any colocalization with the cell membrane stain. While CLN3 internalized, it also co-localized with cell membrane stain. EGFR/c-Met bsApt showed both surface staining and fluorescent intracellular puncta, and its total cell staining was greater compared to monospecific aptamers (MinE07 and CLN3).
    Cellbrite Steady 488 Membrane Staining Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellbrite+steady+488/pmc10589377-418-4-10
    Average 86 stars, based on 1 article reviews
    cellbrite steady 488 membrane staining kit - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    EGFR/c-Met bsApt stains the cell surface and is internalized into H1975 cells Confocal microscopy images of H1975 cells stained upon incubation with 150 nM Atto647N-labeled aptamer samples: C36 (non-targeting control RNA aptamer), MinE07 (anti-EGFR aptamer), CLN3 (anti-c-Met aptamer), or EGFR/c-Met bsApt (bsApt). Cells were also stained with CellBrite Steady 488 (cell membrane stain) and Hoechst 33342 (nuclear marker stain). Representative images from one of n = 2 independent experiments are shown: Atto647N-aptamers (red, excitation 647 nm/emission 657–692 nm), cell membrane (green, excitation 488 nm/emission 521–551 nm), nuclei (blue, excitation 405 nm/emission 436–473 nm). Scale bars, 20 μm. Qualitatively, MinE07 was internalized after 45-min incubation at 37°C and found predominantly in the cytosol, as it did not show any colocalization with the cell membrane stain. While CLN3 internalized, it also co-localized with cell membrane stain. EGFR/c-Met bsApt showed both surface staining and fluorescent intracellular puncta, and its total cell staining was greater compared to monospecific aptamers (MinE07 and CLN3).

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Targeting lung cancer with clinically relevant EGFR mutations using anti-EGFR RNA aptamer

    doi: 10.1016/j.omtn.2023.102046

    Figure Lengend Snippet: EGFR/c-Met bsApt stains the cell surface and is internalized into H1975 cells Confocal microscopy images of H1975 cells stained upon incubation with 150 nM Atto647N-labeled aptamer samples: C36 (non-targeting control RNA aptamer), MinE07 (anti-EGFR aptamer), CLN3 (anti-c-Met aptamer), or EGFR/c-Met bsApt (bsApt). Cells were also stained with CellBrite Steady 488 (cell membrane stain) and Hoechst 33342 (nuclear marker stain). Representative images from one of n = 2 independent experiments are shown: Atto647N-aptamers (red, excitation 647 nm/emission 657–692 nm), cell membrane (green, excitation 488 nm/emission 521–551 nm), nuclei (blue, excitation 405 nm/emission 436–473 nm). Scale bars, 20 μm. Qualitatively, MinE07 was internalized after 45-min incubation at 37°C and found predominantly in the cytosol, as it did not show any colocalization with the cell membrane stain. While CLN3 internalized, it also co-localized with cell membrane stain. EGFR/c-Met bsApt showed both surface staining and fluorescent intracellular puncta, and its total cell staining was greater compared to monospecific aptamers (MinE07 and CLN3).

    Article Snippet: Cells were stained with CellBrite Steady 488 Membrane Staining Kit (Biotium, Fremont, CA) for 1 h at 37°C and 5% CO 2 and then washed twice with DPBS.

    Techniques: Confocal Microscopy, Staining, Incubation, Labeling, Membrane, Marker